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KMID : 1134820000290010106
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 1 p.106 ~ p.110
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Active Oxygen Scavenging Activity of Luteolin-7-O-β-D-Glucoside Isolated from Humulus japonicus
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Park Seung-Woo
Chung Shin-Kyo
Park Jong-Cheol
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Abstract
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We investigated the active oxygen scavenging activity and active compound from Humulus japonicus. The ethyl acetate and butanol fraction exhibited strong scavenging effects on hydroxyl radical. Fur- thermore active compound was isolated and purified using Amberlite XAD-2 column chromatography and preparative HPLC from ethyl acetate fraction, and major compound was identified as a luteolin-7-O-β -D-glucoside by the MS, UV, ¹H-NMR and ¹³C-NMR analyses. Luteolin-7-O-β-D-glucoside exhibited strong scavenging effect on active oxygens such as superoxide radical, hydroxyl radical and hydrogen peroxide.
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KEYWORD
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Humulus japonicus, active oxygen scavenging activity, luteolin-7-O-$\beta$-D-glucoside
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